Abstract

Antimicrobial Activity and Biomedical Application of Sambucus wightiana Phenolic Extract against Gram Positive and Gram-Negative Strains of Bacteria

Increase in drug resistant pathogens has led a way for the development of new drugs by using herbal and medicinal plants extracts. In this study antimicrobial effect of methanolic and ethanolic extract of Sambucus wightiana was recorded positive against 9 pathogenic strains. Extracts of medicinal plant Sambucus wightiana possess cure for many diseases such as extract from ripe fruit cure chronic rheumatism, neuralgia and sciatica. Whole plant extract help in clearing skin and sought out skin problems and is locally used for curing fever, rheumatism and gout. The main purpose of this investigation was to evaluate antimicrobial activity of phenolic extracts of Sambucus wightiana against medically important bacterial strains. Antibacterial activity of whole plant’ s methanolic and ethanolic extract of S. wightiana was checked against 9 bacterial strains including gram positive and negative via agar well diffusion assay method. Antimicrobial activity has been tested against Escherichia coli, Salmonella typhi, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Vibrio cholerae, Enterobacter coleacae, Enterococcus faecalis, Shigella. The result of present study suggests that extract of Sambucus wightiana can be used for treating diseases caused by the tested microorganisms. The ethanolic extract were more potent then methanolic against both gram negative and gram-positive strains. The activity of collected plants was investigated against bacterial strains that cause UTI, GTI, RTI allergies and Skin diseases. The collected medicinal plant was used in folk medicine for treatment of health-related problems. E. coli and S. typhi were most susceptible to methanolic extract while E faecalis was least susceptible to methanolic extract. P. aeruginosa, S. typhi and E. coli were most susceptible to ethanolic extract of S. wightiana.


Author(s):

Najamul Hassan, Hamidullah Shah*, Muhammad Junaid, Sajjad Ali Shah, Shah Faisal, Abdullah, Muhammad Taj Akbar, Adil Khan, Imran Ullah, Adnan Khan, Faheem Jan, Muhammad Waqas, Sajid Iqbal, and Mahmood Khan



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