Assessment of the Osteogenic Potential of Morphogenetic Protein-2 and Insulin-like Growth Factor-I on Adipose Tissue-Derived Stem Cells

Background Stem cells have been identified as the quintessential cell type for regenerative medicine. This study investigates the effects of bone morphogenic protein-2 (BMP-2) and insulin-like growth factor 1 (IGF-1) on bone differentiation. Methods and Findings-de Adipose tissue derived stem cells (ADSCs) were isolated from fat tissue excised from the inguinal region of a healthy white albino rat. The excised tissues were treated and cultured in special media. Several growth factors were used to differentiate stem cells into osteogenic, adipogenic, neurogenic or chondrogenic lineages, and the effects of BMP-2 and IGF-1 on osteogenic differentiation were assessed. Following isolation and culture, the ADSCs had a

spindle-shaped appearance and maintained their mesenchymal-like appearance, indicating an undifferentiated state in a conventional culture medium. The cells began to form colonies from the 10th day of culture and maintained their proliferative capability when cultured in ordinary basic culture medium. When grown in BMP-2 (100 ng/mL) or IGF-1 (200 ng/mL) alone, ADSCs continued to
proliferate. No obvious change in cell morphology was detected under inverted light microscope. Also, no scattered nodules were detected in the field. Alizarin red stain revealed positive effect of combined use of growth factors on ADSCs differentiation.
Conclusion Combined BMP2 and IGF-1 have an osteotropic effect on ADSCs.


Wael Youssef Elias

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