Aim: The aim of the study was to investigate the histological changes of rat epididymis with the effect of cryopreservation on 15, 30, 45 and 60 days freezingthawing.
Methods: The study was performed on male rats, epididymides collected were immediately subjected to 15, 30, 45 and 60 days freezing process, after completion of preservation time they were thawed at 37°C for 10’ were fixed in formalin for histological investigation.
Results: After15, 30, 45 and 60 days of cryopreservation rat epididymis showed reduce in number of sperm in lumen, degenerative changes in tissue shows elongated tubules and loss of epithelium and connective tissue.
Conclusion: In conclusion, the histological changes obtained after freezing storage were reasonable; however, the cryopreserved tubules sheets could not maintain their original layered structure. This work needs to be done to better preservation of the rat epididymis.
Thyagaraju Kedam, Kamala Katepogu and Eswari Beeram