Abstract: Meningioma is the most common primary brain tumor. In spite of the available surgical options and adjuvant treatment, tumor recurrence is still a challenge. Advances in molecular cell biology and genomic techniques have demonstrated in human brain tumors the existence of subpopulations of cells that functionally behave as tumor stem cell. Our knowledge about meningioma molecular pathogeneiticity is still scarce. Knowledge of the cell of origin and mechanism of growth in meningioma offers new opportunity for more effective treatment of the disease.
This study aimed to isolate putative mesenchymal stem like cells from meningioma specimen and to detect in-vivo its tumori-genicity into meningioma.
Methods: Meningioma specimens were processed through two lines, one for RNA extraction and the other for isolation of mesenchymal stem-like cells. CD44, CD73 and CD105 were detected by PCR. Cell surface markers antigens CD34, CD13 and HLA DR were detected by flow cytometry in the isolated MScs. Cultured isolated MSCs were implanted in albino Wister rats’ brain to detect its tumori-genicity into meningioma. Histopathology and immunohistochemistry analysis was used for identification of the growing tumor.
Results: Spindle-shaped and adherent cells similar to MSCs were isolated and successfully cultured. The isolated cells which encoded the cells of origin, demonstrated the ability to differentiate into fibrous meningioma in albino Wister rats brain.
Mohamed A.R. Arbab1,2* Sawsan A. H. Aldeaf1, Alsadig Gassoum1,4, Nihad Elsadig1, Salma Hussin1,3, Nahla E. Abdelrahem1, Hadab A. Mohamed1,2, Lamyaa A.M Elhassan1, Imad Fadl Elmula1,5 and Montaser Altyeb Ibrahim6