Abstract Â Title: Prevalence of bacterial infection among hospital traumatic patients in relation to ABO blood group. Background: There are many studies demonstrated a correlation between blood group antigens and susceptibility to infectious diseases such as bacteria, parasites and viruses. Objectives: To assess the prevalence of bacterial infection among patients in the trauma hospital, and to assess the susceptibility of ABO blood groups to the isolated bacteria. Â Methods and Findings: 166 samples included, wound swabs, sputum and midstream urine were received for routine culture diagnostic procedures from the in-patients at Abosleem Traumatic Hospital and ABO group was obtained from Blood bank documented system for each patient. A correlation between isolated organisms and ABO system was determined.Â 51% patients were infected during their stay in the hospital by one of the following isolates: Pseudomonas (22%); Klebsiella (9%); Staphylococci (15%); and Streptococci (4%). The majority of in-patients belong to blood group O (45%), preceded by group A (37%); B (14%) and AB (4%). The distribution of different blood group within four main bacterial isolates was determined as following: 43% of blood group A patients were susceptible to pseudomonas; (27%) Klebsiella; (36%) Staphylococci; and (29%) Streptococci. While, group B individuals were infected with these organisms: 16% with Pseudomonas, 13% with Klebsiella, 8% with Staphylococci, and 29% with Streptococci.Â Meanwhile, blood group O patients had following strains: 38% Pseudomonas, 53% Klebsiella, 52% Staphylococci, 42% Streptococci. Only, 3% of Pseudomonas, 7% of Klebsiella, and 4% of Staphylococci infected in-patients who expressed blood group AB. Conclusion: The most common bacterial infection among traumatic patient was Pseudomonas, Staphylococci and Klebsiella respectively and individuals of blood group A were found more susceptible to Pseudomonas infection while blood group O more susceptible to Klebsiella and Staphylococci among traumatic patient.