Background: Based on the theoretical basis of the pathological mechanism of cerebral ischemic injury, from the molecular level, using PC12 cells in vitro cell model pharmacodynamics and PK-PD correlation model research, to explain NaomaitongNMTpharmacodynamic material basis for the treatment of cerebral ischemic injury.
Methods: Four in vitro cell models of cerebral ischemic injury were established. The side-leakage of lactate dehydrogenase (LDH) and NO content were used as pharmacodynamic indicators to establish the corresponding drug-time curves of NMT and each single-drug; Based on the SVM algorithm, a PK-PD correlation mathematical model was established.
Results: The cerebral ischemia model caused by NMT has obvious protective effects compared with each single herb. Each single herb has different protective effects on the four damage mechanisms. The protective effect of Radix Pueraria(RP) was stronger in cerebral ischemic injury induced by Glu, In the cerebral ischemic injury caused by calcium overload mechanism, the protective effect of Ligusticum chuanxiongLCwas greater than that of Panax Ginseng(PG) and RP. The 15 components in NMT contained drug serum had a certain correlation with the cell injury model caused by the four mechanisms. It may be a medicinal material for NMT, and the different components have different correlations for cerebral ischemia damage caused by different mechanisms, and each component also has different efficacy.
Conclusions: It reveals the medicinal substance basis of NMT in treating cerebral ischemia,and provides reference for the clinical rational use of NMT for the treatment of cerebral ischemic injury, and provides guidance for the basic research on the substance of traditional Chinese medicine.
Ke-ning Li, Xu-lei Fan, Lan-fang Tan, Sheng-wang Liang, Lei Chen and Shu-mei Wang